Washing FIlm in dark room....

Yesterday, i did sth very funny in the dark room. It was my first time to wash film. That why my senior who guide me in my FYP accompany me to do it.

It was the first time that i did thing in dark. It was like blind. I can only make sure the things that i wanted to use was put according to sequence and feel it with my both gloved hand. Scissors, forceps, timer and a pack of film.

I learn to feel it with my hand when it is dark.... I take a pieces of film out of the package and put it in a cassette. After wash it with developer, H2O and fixer. Light was switched on.

Oppssssssss........... Sth happened....... I'm not holding a film... but a hard paper..... haha.......... I took wrong thing out of the pack of film. The pack of film do protected by a paper and i took the wrong thing....

I have to repeat the process again.........wasting my time... =(
Thanks to my patient senior..... haha.......

WEll, its sth memorable to me.... that happen on the very last day of the year 2009.... haha.....

frustrated...

Recently, I'm so frustrated... I got exam and yet my experiment still din achieve anything. I feel so frustrated till I want to quit. I know it is so silly to say like this.... but I'm really frustrated... My FYP's experiment always give me fake hope... It give me hopes and later it kills me by giving nothing... I hate it... I wish it is over dy but it seems like i'm going to stuck there forever...

Why I work so hard for it and yet I still din get it??? I even go lab everyday during study week just to hope that I get the result that I want for my experiment and yet it disappointed me... It's not only disappointed me, it also make me so frustrated... Due to that, I can't concentrate on my study..

Why is it so hard for CUtinase just to appear??? It makes me feel so so so terrible...... and extreme sad... I follow exactly how the protocol works and yet the Cutinase is so so so stubborn till it doesn't want to appear in the clone. This whole semester, I'm just doing for the same thing. REpeat and repeat..... This FYP's experiment is like killing me slowly...

Digusting virology experiment!!!

This is the embrio taken from the egg that incubated for 8 to 10 days... The two black dots are the eyes of the chick. And that indicate the head too.. The head is bigger than the body. The body is around the size of the head nia.....

What we did to the embrio taken from the egg is to separate the head, wings and legs from the embrio. Then mince the embrio into very very small pieces...... Kinda disgusting but still have to do it.....

This is the head of the embrio left in the petri dish.... YUCKS!!!!!
Poor little chicken embrio....

This is the yolks, allantois fluid..... Very very disgusting......

This is the leftover after the minced body of the embrio in solution..... This the cell debris of the embrio.....

Hopefully u won't feel disgusting after reading it and still able to have ur dinner... hehe.... =P

LAB

Today is an unlucky day..... TERRIBLE........ The lab master student (those who surname is Ma, Mxlxy) really make ppl feel awkward.... So pretending.... In font of us, pretending to help us....

Today, when our phD came and we ask him sth while doing experiment... Those Mxlxy's girls stick in a group and so pretending.

XXXXX : Siapa yang sepatutnya berada di lab bawah???
pHD student answer for us : She is from downstair lab and the other is from upstair....

XXXXX : Siapa yang berada di lab bawah tak boleh buat kerja kat sini. Turun ke bawah buat kerja... Sebab sini dah ada ramai orang..... Mana tempat kamu LiYung???

Crazy ar..... You guy always stay on my bench then I can't even do my own thing..... And the worst thing is they say it in front of the phD student that guide us....

We stick together is because we want to proceed our work together. It doesn't mean we want to do that. And we are not taking the place for whole day.... Really hate them..... So pretending..... In front pretend to be nice and at the back so evil...... Everytime after using this and that, the one that din wash those thing were them, not us.... They even say put more thing on the bench so that we can't use their bench....... Because our experiment are not related to their work, so they can say that to us........ They were so selfish till after using the thing, they din throw away and left the thing there....... Later, those who get blamed were us...... REALLY HATE THEM.........

argh.....

Oh, no...... 3 weeks to go before holiday and my experiment still on the first part..... Polymerase Chain Reaction ar..... Why is it so hard for me to obtain a full length cutinase gene???? Why is it so hard for you to appear nicely in the pic.... haiz....... =(

I'm tired of the experiment dy... can the band of the full length cutinase gene appear in the pic???? Why my thesis experiment so hard to work??????? =(

My megaprimer in the 3rd well....... at 55 degree ceicus

Megaprimer in the first 5 well to be purified......

an unclear band in 3rd well is my cutinase gen at 55 degree celcius....
but it's too little for me to continue to the next step..... sob sob sob.....
for the following experiment, it didn't show up again (no band)...... =(

ARgh!!!!!! Cutinase Gen..... I need u......

haiz....

I'm tired...
Is there any single day that I no need to do homework or plan for my experiment???
Is there any single day that I no need to go to lab???
Please just give me a rest????

Since the starting of 3rd year....
There is never a day without homework or anything related to experiment???
Couldn't I just have relax day.....
3rd year is always surrounded by homework, proposal, thesis, experiment.....
Besides that... there is also quiz...
argh!!!! Going to be crazy soon.....
It's tiring and sleepy.....

No matter how, I had to overcome this....
This is the final year and soon it will be over....
Just have to be patient with it.....
Haiz.......

thesis Proposal Outline

Anyone know how to write Proposal outline?? If you have any proposal sampel, please give me a copy... I'm desperate to have one...

My thesis tittle is "Pengekspresan dan pencirian varian kutinase L198A"... I'm still blur with it.. I dunno what to do... The thing that I can do now is to read, read and read..... Soon my spec degrees will increase... haha.....

Cutinase can partially degrade PET. The plastic bottle that can only be used once. Cutinase come from a type of fungus named Glomerella cingulata. How it looks like i also have no idea... This is what I know so far... anyway thanks to the PhD student that dun mind to explain the experiment to us and give us lots of reading material.....

Now just hoping I can write the proposal outline in time... argh..... left 3 days and i got to pass up...